Review



grna screening vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Addgene inc grna screening vector
    a , Overview of <t>gRNA</t> assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a <t>non-functional</t> <t>EGFP</t> that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Grna Screening Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grna screening vector/product/Addgene inc
    Average 96 stars, based on 121 article reviews
    grna screening vector - by Bioz Stars, 2026-04
    96/100 stars

    Images

    1) Product Images from "Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing"

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing

    Journal: Nature Methods

    doi: 10.1038/s41592-025-02805-0

    a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Figure Legend Snippet: a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Techniques Used: Binding Assay, Functional Assay, Flow Cytometry



    Similar Products

    grna screening vector  (Addgene inc)
    96
    Addgene inc grna screening vector
    a , Overview of <t>gRNA</t> assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a <t>non-functional</t> <t>EGFP</t> that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Grna Screening Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grna screening vector/product/Addgene inc
    Average 96 stars, based on 1 article reviews
    grna screening vector - by Bioz Stars, 2026-04
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Journal: Nature Methods

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing

    doi: 10.1038/s41592-025-02805-0

    Figure Lengend Snippet: a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Article Snippet: The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA .

    Techniques: Binding Assay, Functional Assay, Flow Cytometry